This research demonstrates the design of Joyful Mobile Navigation Systems (JMNS), which consists of joyful, mobile, flexible and ubiquitous features. The Technology Acceptance Model (TAM) and Integrating Learning Models (ILM) were used to design the teaching activities of Flow Learning and Inquiry-Based Learning in order to enhance learning motivation and interests for students in the ecological teaching activity. The developed JMNS belongs to a personalized informatin station of KIOSK that contains RFID bar-code recognization, tag reader, input device, mobile computation unit, QRcode, mobile display device, wireless local network and operating software. The aim of this research is to design and analyze the JMNS’s characteristics of the external variables, reliability and validity by measuring through the Human-Computer interface, which is achieved through the Questionnaire for User Interface Satisfaction (QUIS) and implemented by using QR Code, GPS and RFID technologies. The developed JMNS have been applied in real teaching activity for improving learning intention. The research results show that the designed JMNS and teaching activity have a positive significant impact on learning motivation, material characteristics, perceived usefulness, perceived ease of use, attitude to use, and behavioral intention. The results also show that only significant of part with support for the students’ system characteristics to the perceived usefulness, and perceived ease of use. That is, system developers should improve the design of Context Awareness System of teaching to help students in mobile navigation and teaching activity to have a comfortable interaction and increased learning intention. Furthermore, using JMNS in teaching activity can simultaneously increase learners’ motivation and offer a more joyful perception to learners during the learning process.